Description
Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
Form
White powder, lyophilized
Enzyme Commission Number
EC 2.7.1.40
Activity
>200U/mg protein
Molecular Weight
68 kDa (SDS-PAGE)
pH Stability
5.0~10.0 (37℃, 20hr)
Michaelis Constant
1.1mM (ADP)
2.2mM(PEP)
Unit Definition
One unit will convert one micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30℃.
Thermal stability
< 60℃ (pH 8.5, 20min)
Inhibitors
Ag+, Hg2+, Co2+, Fe3+
Synonyms
EC 2.7.1.40; 9001-59-6; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; pyruvate kinase (phosphorylating); fluorokinase; fluorokinase (phosphorylating); pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase
Notes
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
