Products

Enzymes for Research, Diagnostic and Industrial Use

Products
Online Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

24 hour
Promise

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.

Transglutaminase 2 from Rabbit, Recombinant

Cat No.
NATE-1734
Description
This enzyme is based on the NCBI database sequence XM_008256006. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHAEDLIL…. This enzyme is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.
Abbr
TG 2, Recombinant (Rabbit)
Alias
TG2
Source
E. coli
Species
Rabbit
Applications
The transglutaminase 2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
The transglutaminase 2 may also be used for immunoprecipitation.
Appearance
White lyophilized solid.
Package
250 μg; 1mg
Form
The enzyme is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8.0.
Sample contains maltodextrin.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231)].
CAS No.
80146-85-6
Molecular Weight
77 kDa
Purity
> 95 % (visually by SDS-PAGE)
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 10 nm; detector strength = 600 V; temperature = 37 °C, assay volume = 1 ml)].
Activators
Add 10 mM Ca2+ to activate transglutaminase.
Storage
Store at -20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Delivery is possible at ambient temperature.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
Reconstitution
Add the volume of H2O the protein is lyophilized from to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
After reconstitution, the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.

"Transglutaminase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1737 Transglutaminase 7 from Human, Recombinant EC 2.3.2.13 80146-85-6 E. coli Inquiry
NATE-1725 Biotinylated Transglutaminase from Human, Recombinant EC 2.3.2.13 80146-85-6 Insect cells Inquiry
NATE-1724 Transglutaminase 1 from Human keratinocyte, Recombinant EC 2.3.2.13 80146-85-6 Insect cells Inquiry
NATE-1723 Transglutaminase 1 from Human keratinocyte, Recombinant EC 2.3.2.13 80146-85-6 E. coli Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

0
Click unfold / close
Inquiry Basket
Delete selected Quote Check Out
Decide to move out of the shopping cart?
Sure No, Back

Please choose product!

< Go Back
You have already added to buy this product