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Transglutaminase from Guinea pig, Recombinant

Cat No.
NATE-1721
Description
Catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
Abbr
TGase, Recombinant (Guinea pig)
Source
E.coli
Species
Guinea pig liver
Form
Lyophilized from 50mM NaH2PO4, pH 8.0, containing 150mM sodium chloride. Sample contains maltodextrin.
Enzyme Commission Number
EC 2.3.2.13
Activity
>8 U/mg
CAS No.
80146-85-6
Molecular Weight
~77kDa
Purity
>95% (SDS-PAGE)
Unit Definition
One unit is defined as the formation of 1 μmol hydroxamate per minute from Z-Gln-Gly-OH and hydroxylamine at pH 6.0 at 37°C containing 10mM CaCl2 (L-Glutamic acid γ-monohydroxamate is the standard)].
Storage
Store at -20°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Reconstitution
Reconstitute with water. Rotate vial gently until solid dissolves.
Notes
INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
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Related Protocols
transglutaminase -Enzymatic Assay Protocol
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