Description
Unsaturated rhamnogalacturonyl hydrolase (EC 3.2.1.172, YteR, YesR) is an enzyme with systematic name 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose hydrolase. This enzyme catalyses the following chemical reaction: 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O → 5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose.
Abbr
YteR 105A, Recombinant (Bacillus subtilis)
Species
Bacillus subtilis
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.172
Molecular Weight
43.4 kDa
Purity
>90% as judged by SDS-PAGE
Specificity
Unsaturated rhamnogalacturonan (RG)
Optimum temperature
30 °C
Storage
This enzyme is shipped at room temperature but should be stored at -20 °C.
Synonyms
Unsaturated rhamnogalacturonyl hydrolase; EC 3.2.1.172; YteR; YesR
