alpha-2,6-Sialyltransferase (Pd26ST)

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Cat No.

ASGZ-0001

Description

alpha-2,6-Sialyltransferase (Pd26ST) transfers sialic acid to terminal or internal galactose and N-acetylgalactosamine residues, producing terminal α2,6-linked sialosides.

Abbr

Pd26ST

Alias

alpha-2,6-Sialyltransferase; beta-Galactoside alpha-2,6-Sialyltransferase

Source

Recombinant Escherichia coli expression

Species

Pasteurella multocida (P-1059)

Applications

Enzymatic synthesis of α2,6-sialylated glycans and glycoconjugates; modification of galactose- or N-acetylgalactosamine-containing acceptors.

Appearance

Colorless to light yellow liquid

Product Overview

This recombinant C-terminal His-tagged enzyme is supplied as a liquid preparation. It has an approximate molecular weight of 54 kDa, a purity greater than 90% by SDS-PAGE, and an enzyme activity of at least 5 U/mL.

Form

Liquid

Enzyme Commission Number

2.4.99.1

Activity

≥5 U/mL

CAS_No

9075-81-4

Molecular Weight

Approximately 54 kDa

Purity

>90% by SDS-PAGE

Specificity

Accepts terminal and internal galactose and N-acetylgalactosamine residues and forms terminal α2,6-sialosides.

Unit Definition

One unit is defined as the amount of enzyme required per minute to convert 1 μmol of Neu5Ac from UDP-Neu5Ac to lactose to form Neu5Ac-α2,6-lactose at 37°C.

Storage

Store under the conditions specified in the batch Certificate of Analysis.

Stabilizers

10% glycerol

Buffer

20 mM Tris-HCl, pH 8.0

Preparation Instructions

Add the required substrate, 5 mM MgCl₂, and an appropriate buffer such as Tris-HCl. Adjust the reaction mixture to pH 8.5, add the required amount of enzyme, mix, and incubate at 37°C for 2-4 hours.

Warnings

For scaled reactions, adjust the enzyme amount or incubation time according to the actual reaction system. Low-speed shaking may be used to maintain mixing.

Function

Catalyzes formation of terminal α2,6-linked sialosides.

Synonyms

Pd26ST; alpha-2,6-Sialyltransferase; beta-Galactoside alpha-2,6-Sialyltransferase

Substrates

Terminal or internal galactose and N-acetylgalactosamine-containing acceptors

Quality

Recombinant enzyme; purity >90% by SDS-PAGE; activity ≥5 U/mL

Reaction

Transfers sialic acid to galactose or N-acetylgalactosamine acceptor residues to generate terminal α2,6-linked sialosides.

Notes

The source page identifies the product as Pd26ST and lists Pasteurella multocida (P-1059) in the product title. Product identity and activity should be confirmed using the batch documentation.

Protocol

1. Add the substrate required for the reaction system.
2. Add MgCl₂ to a final concentration of 5 mM.
3. Add a suitable buffer, such as Tris-HCl, and adjust the reaction to pH 8.5.
4. Add an appropriate amount of alpha-2,6-Sialyltransferase.
5. Mix and incubate at 37°C for 2-4 hours.
6. For scale-up, adjust the incubation time or enzyme amount according to the reaction system; low-speed shaking may be used for mixing.

Usage

Use as a recombinant glycosyltransferase for preparation of terminal α2,6-sialylated products. Select the enzyme amount according to substrate loading and the required conversion.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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