Arginine Specific Protease from Porphyromonas gingivalis

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Cat No.

NATE-1602

Description

Arginine Specific Protease specifically digests peptides and proteins C-terminal to arginine residues. The protease is specific for Arg-X motifs and does not have activity at lysines as commonly observed using Arg-C. The enzymatic activity of Arginine Specific Protease includes digestion of Arg-Pro linkages, which is difficult to digest with other enzymes. The enzyme is active at pH ranging from pH 5.0-9.0 and in presence of denaturing agents such as 6M urea and 0.1% SDS.

Abbr

Arginine Specific Protease (Porphyromonas gingivalis)

Source

Porphyromonas gingivalis

Applications

Peptide mapping
Protein sequence analysis
Protein fingerprinting
Post-translational modification analysis

Appearance

White to light yellow powder

Form

Lyophilized powder in 20 mM Bis-Tris, 150 mM NaCl, 5 mM CaCl2, Ph 6.8 without preservatives

Purity

> 95% homogeneity as determined by SDS-PAGE and HPLC.

pH Stability

5.0-9.0

Optimum pH

6.5-8.0

Stability

1 year when stored at -20°C. After reconstitution by addition of water it is stable for at least 1 month at 4-8°C.

Storage

The product is shipped on ice and should be stored at -20°C upon arrival.

Buffer

To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment. Arginine Specific Protease is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween, SDC. Buffers tested and compatible with Arginine Specific Protease activity are Tris, Bis-Tris and ammonium bicarbonate.

Synonyms

Arginine Specific Protease; GingisREX

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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