Beta Lactamase from E.coli, Recombinant

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Cat No.

NATE-1886

Description

Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties.

Source

E. coli

Species

E. coli

Form

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-7.

Enzyme Commission Number

EC 3.5.2.6

Activity

700IU/mg

Molecular Weight

29 kDa

Purity

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25°C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Stability

Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms

b-Lactamase; EC 3.5.2.6; TEM precursor; β-lactamase

Solubility

It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18M?-cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0- 8.0 and in temperature not higher then 45° C.

"β-Lactamase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1839	Lactamase (Crude Enzyme)	EC 3.5.2.6	9073-60-3	E. coli	Inquiry
NATE-0542	Native Bacillus cereus Penicillinase	EC 3.5.2.6	9073-60-3	Bacillus cereus	Inquiry
NATE-0774	Native Enterobacter cloacae β-Lactamase	EC 3.5.2.6	9073-60-3	Enterobacter cloacae	Inquiry
NATE-0775	Native Pseudomonas fluorescens β-Lactamase	EC 3.5.2.6	9073-60-3	Pseudomonas fluorescens	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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