Bovin beta-1,4-galactosyltransferase 1 (Y289L)

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Cat No.

BBGYG-0001

Description

Bovin beta-1,4-galactosyltransferase 1 (Y289L) is a mutant galactosyltransferase with broadened donor-substrate specificity that transfers GalNAc or GalNAz to terminal GlcNAc residues.

Abbr

B4GALT1(Y289L)

Alias

Bovin B4GALT1 (Y289L); GalT Y289L; Y289L beta-1,4-Galactosyltransferase 1

Source

Recombinant HEK293 cell expression; C-terminal His tag

Species

Bos taurus (bovine)

Applications

Chemoenzymatic labeling of O-GlcNAcylated proteins; glycan engineering; preparation of azide-functionalized glycoconjugates for downstream click-chemistry analysis.

Appearance

Colorless to light yellow liquid

Package

100 µg
1 mg
5 mg
10 mg
50 mg

Product Overview

This HEK293-expressed, C-terminal His-tagged Y289L mutant has a molecular weight of 50.8 kDa and a specific activity of 2500 U/mg. It is supplied in 20 mM Tris-HCl (pH 7.4), 200 mM NaCl, and 20% glycerol.

Form

Liquid

Molecular Weight

50.8 kDa

Specificity

Transfers GalNAc or GalNAz to terminal GlcNAc residues.

Unit Definition

One unit is defined as the amount of enzyme that transfers 1 nmol of GalNAz from UDP-GalNAz to GlcNAc per minute at 37°C.

Activators

Mn²⁺

Storage

Store under the batch-specific recommended conditions.

Stabilizers

20% glycerol

Buffer

20 mM Tris-HCl, 200 mM NaCl, pH 7.4

Function

Transfers GalNAc or GalNAz to terminal GlcNAc residues and introduces a reactive azide handle when UDP-GalNAz is used as the donor.

Synonyms

Bovin B4GALT1 (Y289L); GalT Y289L; Y289L beta-1,4-Galactosyltransferase 1

Specific Activity

2500 U/mg

Reaction

UDP-GalNAz + terminal GlcNAc acceptor → GalNAz-labeled product + UDP

Notes

The Y289L substitution broadens donor-substrate acceptance to GalNAc and GalNAz.

Protocol

1. Prepare the reaction system with 50 mM Tris-HCl (pH 7.5) and 6 mM MnCl₂.
2. Use a UDP-GalNAz-to-antibody equivalent ratio of 300:1.
3. Set the enzyme-to-antibody addition amount to 5%.
4. Incubate the reaction at 37°C for 48 hours.
5. Transfer the reaction to -25°C to -15°C to stop the reaction, then proceed with sample analysis.

Usage

For chemoenzymatic labeling of O-GlcNAcylated proteins and glycan-modification workflows.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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