Calmodulin-dependent Protein Kinase II from Rat, Recombinant

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Cat No.

NATE-1273

Description

Serine-threonine protein kinase:these kinases appear to be involved in neurotransmitter release, control of stimulus-induced gene expression, and in the phosphorylation of microtubule related proteins.

Abbr

CaMKII, Recombinant (Rat)

Source

S. frugiperda Sf9

Species

Rat

Product Overview

Calmodulin-Dependent Protein Kinase II (CaMKII) is a serine/threonine kinase. It is a Ca2+/calmodulin-dependent, truncated monomer (1-325 amino acid residues) of the α subunit. Autophosphorylation of threonine 286 in the presence of Ca2+ and calmodulin activates CaMKII and produces substantial Ca2+/calmodulin-independent activity.

Form

100 mM NaCl, 50 mM HEPES (pH 7.5 25°C), 0.1 mM EDTA, 1 mM DTT, 0.01% Brij 35 and 50% glycerol.

Activity

5,000,000 units/mg

Molecular Weight

Apparent: 33 kDa Theoretical: 36 kDa

Purity

> 95% determined by SDS-PAGE

Concentration

500,000 units/ml

Unit Definition

One unit is defined as the amount of activated CaMKII required to catalyze the transfer of 1 pmol of phosphate from ATP(200mM) to Autocamtide-2 (CaMKII Peptide Substrate), KKALRRQETVDAL (50 µM), in 1 minute at 30°C in a total reaction volume of 30 µl.

Storage

at -70°C. Avoid repeated freeze/thaw cycles.

Synonyms

Calmodulin-dependent Protein Kinase II; 9026-43-1

"Calmodulin-dependent Protein Kinase II" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0099	Native Rat Calmodulin-dependent Protein Kinase II		9026-43-1	Rat brain	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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