CHIR-99021 (CT99021)

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Cat No.

CEI-1279

Description

CHIR-99021 (CT99021) is an inhibitor of GSK-3α/β with IC50 of 10 nM/6.7 nM; > 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases.

Product Overview

CHIR-99021 is a highly specific glycogen synthase kinase-3 (GSK-3) inhibitor which can inhibit both isoforms with IC50 of 10 nM (GSK-3α) and 6.7 nM (GSK-3β). CHIR-99021 was proved to promote self-renewal and maintain pluripotency of both B6 and BALB/c ES cells via stabilizing the downstream effectors like c-Myc and β-catenin. In J1 mESC cells, CHIR-99021 played an important role in maintaining the colony morphology as well as the self-renewal when combined with leukemia inhibitory factor (LIF). CHIR-99021 has shown to regulate multiple signaling pathways which involve Wnt/β-catenin, TGF-β, Nodal and MAPK, and the expression of epigenetic regulatory genes like Dnmt3l. CHIR-99021 has been demonstrated to promote DN3 thymocytes proliferation and differentiation in the absence of pre-TCR signaling, Notch1 signaling or CXCL12.However, study has also found that higher concentration (10 nM but not 1 nM or 3 nM) of CHIR99021 might selectively inhibit differentiation by activating IL-7 signaling pathway.

CAS_No

252917-06-9

Molecular Weight

465.34

Purity

0.9823

Storage

Store at 4°C

Synonyms

CHIR99021, CHIR-99021, CHIR 99021, CT99021,GSK-3 Inhibitor XVI

Targets

GSK-3

IC50

7 nM

Molecular Formula

C22H18Cl2N8

Chemical Name

6-[2-[[4-(2,4-dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)pyrimidin-2-yl]amino]ethylamino]pyridine-3-carbonitrile

Solubility

Soluble in DMSO

Shipping Conditions

Evaluation sample solution: ship with blue ice. All other available size: ship with RT, or blue ice upon request

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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