Creatininase amidohydrolase

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Cat No.

CAGZ-0001

Description

Creatininase amidohydrolase is a microbial hydrolase that converts creatinine to creatine by cleaving a carbon-nitrogen bond other than a peptide bond.

Abbr

CAH

Alias

Creatinine amidohydrolase

Source

Microbial enzyme from Pseudomonas putida

Species

Pseudomonas putida

Applications

Biochemical research and enzyme-based creatinine analysis.

Appearance

White to off-white solid

Package

1 KU

Product Overview

This homohexameric enzyme provides high catalytic activity and broad pH stability. It is suitable for coupled enzyme systems in which the generated creatine is processed in subsequent reactions.

Form

Solid

Enzyme Commission Number

3.5.2.10

Activity

≥400 U/mg

CAS_No

9025-13-2

Molecular Weight

29 kDa (SDS-PAGE)

Isoelectric point

5.3

pH Stability

pH 5.5-10.0 at 25°C for 16 h

Michaelis Constant

Km: 5.0 × 10⁻² M (creatinine); 8.0 × 10⁻² M (creatine)

Structure

Homohexameric enzyme

Specificity

Creatinine

Optimum pH

7.0-8.0

Optimum temperature

65°C

Thermal stability

Stable below 65°C at pH 8.0 for 30 min

Inhibitors

Hg²⁺, Cu²⁺, Fe³⁺

Preparation Instructions

Dissolve in pure water at a concentration below 50 mg/mL, or use an appropriate buffer for the intended experiment. Ultrasonication may be required when preparing a 50 mg/mL aqueous solution.

Function

Catalyzes hydrolysis of creatinine to creatine.

Synonyms

Creatininase; Creatinine amidohydrolase; CAH

Solubility

H₂O: 50 mg/mL; ultrasonication required

Substrates

Creatinine

Reaction

Creatinine + H₂O → creatine

Notes

Creatine is also reported as a substrate for the reverse reaction under the stated kinetic characterization.

Usage

For biochemical studies and the development of enzyme-based creatinine assay systems.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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