Cyclooxygenase 2 from Ovine, Recombinant

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Cat No.

NATE-1239

Description

Cyclooxygenase 2 (COX-2) catalyzes the first step in the biosynthesis of prostaglandins (PGs), thromboxanes, and prostacyclins: The conversion fo arachidonic acid to PGH2. Discoveries of the induction of COX expression by a variety of stimuli such as phorbol esters, lipopolysaccharides, and cytokines led to the hypothesis that the inducible form of COX, COX-2, is responsible for the biosynthesis of PGs under acute inflammatory conditions. Thus, COX-2 has become the focus of attention for the nonsteroidal anti-inflammatory drug (NSAID) development. Human recombinant COX-2 contains a six residue histidine sequence (His-tag) near the amino terminus. The His-tag enzyme, which has a Km value for arachidonate of 6.5 µM, exhibits enzyme activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Abbr

COX-2, Recombinant (Ovine)

Alias

COX-2

Source

Sf21 cells

Species

Ovine

Activity

>20,000 U/mg

Molecular Weight

~70 kDa

Purity

>80% estimated by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme required to consume 1 nmol of oxygen per minute 37°C in 100 mM Tris, pH 8.0, containing 100 µm arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.

Stability

> 6 months

Storage

-80°C (as supplied)

Synonyms

Cyclooxygenase 2; Inducible Cyclooxygenase Prostaglandin H Synthase 2; COX-2

"COX-2" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1238	Cyclooxygenase 2 from Human, Recombinant			Sf21 cells	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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