Diaphorase 22 from Recombinant E.coli

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Cat No.

NATE-1938

Description

Recombinant Diaphorase 2.2 from E. coli for advanced research on redox reactions and enzymatic activity. Ideal for biochemistry and molecular biology studies. Creative Enzymes ensures high-purity, reliable solutions.

Source

E. coli

Form

Lyophilized

Enzyme Commission Number

EC 1.8.1.4

Activity

>150 U/mg protein

CAS_No

9001-18-7

Contaminants

(as Diaphorase activity = 100 %)
Adenylate kinase < 0.01 %
NADH oxidase < 0.20 %

Molecular Weight

ca. 110,000

pH Stability

6.0 – 9.0

Michaelis Constant

(50 mM HEPES buffer, pH 7.0, at 30 °C)
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) 0.345 mM
NADH 0.033 mM

Unit Definition

One unit of activity is defined as the amount of Diaphorase that forms 1 μmol of NAD+ per minute at 30 °C.

Optimum pH

Thermal stability

No detectable decrease in activity up to 70 °C.

Storage

Store at -20°C

Synonyms

LDP-Glc; LDP-Val; dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; protein-6-N-(dihydrolipoyl)lysine:NAD+ oxidoreductase

"Diaphorase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-320	Diaphorase from E. coli, Recombinant	EC 1.8.1.4	9001-18-7	E. coli	Inquiry
NATE-1927	Diaphorase-3, recombinant				Inquiry
NATE-1901	Native Bacillus stearothermophilus Diaphorase 1	EC 1.6.99.-		Bacillus stearothermophilus	Inquiry
DIA-142	Native Bacillus megaterium Diaphorase (NADH)	EC 1.6.99.3	9079-67-8	Bacillus megaterium	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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