Eukaryotic TMP Kinase, Recombinant

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Cat No.

CEGZ-6404

Description

A recombinant mesophilic eukaryotic thymidylate kinase with activity toward TMP, dGMP, and several additional nucleotide monophosphates.

Abbr

TMPK

Alias

dTMPK

Source

Recombinant E. coli

Species

Eukaryotic

Applications

For research and in vitro phosphorylation of dTMP and related purine or pyrimidine monophosphates at mesophilic temperatures.

Product Overview

The enzyme phosphorylates TMP and dGMP and, to a lesser extent, dUMP, dIMP, and GMP as well as modified pyrimidine and purine derivatives. ATP and dATP can act as donors.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.9

CAS_No

9014-43-1

Molecular Weight

26.0 kDa

Specificity

TMP and dGMP; lower activity toward dUMP, dIMP, GMP, and modified pyrimidine and purine derivatives. ATP and dATP can act as donors.

Storage

-20°C

Synonyms

TMPK; thymidine monophosphate kinase; thymidylate kinase; deoxythymidine 5'-monophosphate kinase; dTMPK; dTMP kinase; thymidylic acid kinase; thymidylic kinase; ATP:dTMP phosphotransferase

Substrates

ATP or dATP; dTMP; dGMP; dUMP; dIMP; GMP

Reaction

ATP + dTMP = ADP + dTDP

Notes

Mesophilic enzyme. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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