Eukaryotic UMP Kinase, Recombinant

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Cat No.

CEGZ-6405

Description

A recombinant mesophilic eukaryotic UMP kinase with activity toward UMP, dUMP, and selected purine and pyrimidine monophosphates.

Abbr

UMPK

Source

Recombinant E. coli

Species

Eukaryotic

Applications

For research and in vitro phosphorylation of UMP, dUMP, and related nucleotide monophosphates under mesophilic conditions.

Product Overview

The enzyme phosphorylates UMP and dUMP and, to a smaller extent, GMP, dGMP, and TMP. ATP and dATP can act as phosphate donors.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.22

CAS_No

9036-23-1

Molecular Weight

24.2 kDa

Specificity

UMP and dUMP; lower activity toward GMP, dGMP, and TMP. ATP and dATP can act as donors.

Storage

-20°C

Synonyms

UMPK; uridylate kinase; uridine monophosphate kinase; ATP:UMP phosphotransferase

Substrates

ATP or dATP; UMP; dUMP; GMP; dGMP; TMP

Reaction

ATP + UMP = ADP + UDP

Notes

Mesophilic enzyme. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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