Glucose-6-phosphate Dehydrogenase from E. coli, Recombinant

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Cat No.

DIA-407

Description

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ↔ 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

Abbr

G6PDH, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Form

In 3.2 M ammonium sulphate.

Enzyme Commission Number

EC 1.1.1.49

Activity

172 U/mg

CAS_No

9001-40-5

Molecular Weight

~ 56,770

Concentration

~ 1,250 U/ml

Isoelectric point

~ 5.8

Unit Definition

One Unit of glucose 6-phosphate dehydrogenase (G6PDH) is defined as the amount of enzyme required to produce one µmole of NADPH from NADP+ per minute.

Optimum pH

7.6

Optimum temperature

25°C

Stability

> 2 years

Storage

4°C

Preparation Instructions

For assay, this enzyme should be diluted in 200 mM Imidazole buffer, pH 7.6 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.

Synonyms

EC 1.1.1.49; NADP-glucose-6-phosphate dehydrogenase; Zwischenferment; D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; glucose-6-phosphate 1-dehydrogenase; G6PDH; GPD; glucose-6-phosphate dehydrogenase; 9001-40-5

"Glucose-6-phosphate dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1898	Native Zymomonas mobilis Glucose-6-Phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Zymomonas mobilis	Inquiry
DIA-143	Native Bacillus sp. Glucose-6-phosphate dehydrogenase	EC 1.1.1.49	9001-40-5	Bacillus sp.	Inquiry
DIA-145	Native Microorganism Glucose-6-phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Microorganism	Inquiry
DIA-219	Native baker's yeast (S. cerevisiae) Glucose-6-phosphate Dehydrogenase	EC 1.1.1.49	9001-40-5	Baker's yeast (S. cerevisiae)	Inquiry

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