Glycine Oxidase H244K from Bacillus subtilis, recombinant

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Cat No.

NATE-1674

Description

Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a homotetrameric flavin-dependent oxidoreductase. Each GO monomer is non-covalently bound to flavin adenine dinucleotide. GO catalyzes oxidative deamination of various primary and secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the corresponding α-keto acids and hydrogen peroxide. Primarily, glycine oxidase catalyzes the oxidation of glycine in the biosynthesis of thiamine. The variant H244K shows a higher substrate specificity ratio for glycine versus sarcosine and a 5-fold improved specific activity in comparison to the wild-type.

Abbr

GO, Recombinant (Bacillus subtilis)

Source

E. coli

Species

Bacillus subtilis

Form

Liquid

Enzyme Commission Number

EC 1.4.3.19

Activity

1200 mU/mg

CAS_No

39307-16-9

Molecular Weight

43.1 kDa (1-369 aa, NT His Tag)

Purity

> 90% by SDS-PAGE

Concentration

4.0 mg/ml

Unit Definition

One unit is defined as the amount of enzyme required to convert one micromole of glycine into glyoxylate and hydrogen peroxide at pH 8.5 at 37°C.

Storage

Store at –20°C. Stable for at least 1 year as supplied. Avoid repeated freeze and thaw cycles.

Synonyms

Glycine oxidase; glycine oxygen oxidoreductase (deaminating); GO; EC 1.4.3.19; 39307-16-9

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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