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Granzyme K from Human, Recombinant

Cat No.
NATE-1936
Description
Granzyme K is a protein that in humans is encoded by the GZMK gene. This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.
Source
E. coli
Species
Human
Form
Liquid. In PBS containing 10% sucrose and ~0.5M sodium chloride. Contains no preservatives.
Enzyme Commission Number
EC 3.4.21.-
Activity
~29 U/µg protein
Purity
>98% (SDS-PAGE)
Concentration
0.2 mg/ml
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1nmol Z-Lys-SBzl per min. at 25°C in 0.05M TRIS, pH 8.0, containing 0.15M NaCl, 0.01% Triton X-100 and 0.3mM DTNB.
Stability
Stable for at least 6 months when stored at -80°C. Dilute solutions (e. g. 1-1000ng/ml) should be used within 24 hours.
Storage
at -80°C
Warnings
For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -80°C.
Synonyms
Granzyme K; GZMK; GZMK
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