High-Thermostability Pyrimidine Nucleoside Phosphorylase, 80°C

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Cat No.

CEGZ-6390

Description

A recombinant bacterial pyrimidine nucleoside phosphorylase distinguished by thermal stability up to at least 80°C and a molecular mass of 48.4 kDa.

Abbr

PyNP

Alias

Py-NPase; PYNP

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro use in reversible phosphorolysis and synthesis of pyrimidine nucleosides where elevated-temperature stability is required.

Product Overview

The enzyme accepts uridine, thymidine, and 2-deoxyuridine in the phosphorolytic direction and supports the corresponding pentose-1-phosphate-dependent reverse reactions.

Form

Liquid

Enzyme Commission Number

EC 2.4.2.2

CAS_No

9055-35-0

Molecular Weight

48.4 kDa

Thermal stability

Thermostable up to at least 80°C

Storage

4°C

Synonyms

PyNP; Py-NPase; PYNP; pynpase; pyrimidine ribonucleoside phosphorylase; pyrimidine-nucleoside:phosphate (2'-deoxy)-alpha-D-ribosyltransferase

Substrates

Uridine; 2-deoxyuridine; thymidine; phosphate

Reaction

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
2-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate

Notes

Recombinant protein produced in E. coli and purified by heat treatment and His6-tag affinity chromatography.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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