Isocitrate dehydrogenase from Bacillus subtilis, Recombinant

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Cat No.

NATE-1103

Description

Isocitrate dehydrogenase (IDH) (EC 1.1.1.42) and (EC 1.1.1.41) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and CO2. This is a two-step process, which involves oxidation of isocitrate (a secondary alcohol) to oxalosuccinate (a ketone), followed by the decarboxylation of the carboxyl group beta to the ketone, forming alpha-ketoglutarate. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step of the citric acid cycle while converting NAD+ to NADH in the mitochondria. The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They localize to the cytosol as well as the mitochondrion and peroxisome.

Source

Bacillus subtilis

Form

Liquid

Enzyme Commission Number

EC 1.1.1.42

Activity

~ 13 U/mg protein

CAS_No

9028-48-2

Molecular Weight

~ 48.5kD

Unit Definition

One unit is the amount of enzyme required to convert one µmole of D-/L-isocitric acid to α-ketoglutarate per minute in Tris-HCl buffer at pH 7.6 and 25°C.

Storage

4°C

Synonyms

isocitrate dehydrogenase (NADP+); oxalosuccinate decarboxylase; oxalsuccinic decarboxylase; isocitrate (NADP) dehydrogenase; isocitrate (nicotinamide adenine dinucleotide phosphate) dehydrogenase; NADP-specific isocitrate dehydrogenase; NADP-linked isocitrate dehydrogenase; NADP-dependent isocitrate dehydrogenase; NADP isocitric dehydrogenase; isocitrate dehydrogenase (NADP-dependent); NADP-dependent isocitric dehydrogenase; triphosphopyridine nucleotide-linked isocitrate dehydrogenase-oxalosuccinate carboxylase; NADP+-linked isocitrate dehydrogenase; IDH (ambiguous); dual-cofactor-specific isocitrate dehydrogenase; NADP+-ICDH; NADP+-IDH; IDP; IDP1; IDP2; IDP3

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Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1649	Mutant Isocitrate Dehydrogenase 1 R132H from Human, Recombinant			E. coli	Inquiry
NATE-0350	Native Porcine Isocitric Dehydrogenase (NADP)	EC 1.1.1.42	9028-48-2	Porcine heart	Inquiry
NATE-0351	Isocitrate Dehydrogenase (NADP+) from Yeast, Recombinant	EC 1.1.1.42	9028-48-2	Pichia pastoris	Inquiry
NATE-1029	Isocitrate Dehydrogenase (NADP+) from Bacteria, Recombinant	EC 1.1.1.42	9028-48-2	E. coli	Inquiry

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