Liquid Lactobacillus leichmannii Nucleoside Deoxyribosyltransferase II

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Cat No.

CEGZ-6395

Description

A recombinant type II nucleoside deoxyribosyltransferase from Lactobacillus leichmannii supplied as a buffered liquid containing glycerin.

Abbr

NDT

Alias

DRTase; trans-deoxyribosylase

Source

Recombinant E. coli

Species

Lactobacillus leichmannii

Applications

For research and in vitro one-step deoxyribosyl-transfer reactions between purine and pyrimidine nucleobases.

Product Overview

The enzyme transfers a 2-deoxyribosyl moiety from a 2-deoxynucleoside donor to a nucleobase acceptor and supports broad purine-to-purine, purine-to-pyrimidine, and pyrimidine-to-pyrimidine transfer.

Form

Liquid

Enzyme Commission Number

EC 2.4.2.6

CAS_No

9026-86-2

Molecular Weight

19.3 kDa

Specificity

Broad substrate spectrum; supports purine-to-purine, purine-to-pyrimidine, and pyrimidine-to-pyrimidine transfer.

Storage

-20°C

Synonyms

NDT; nucleoside 2'-deoxyribosyltransferase type II; trans-deoxyribosylase; deoxyribose transferase; DRTase; purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase; nucleoside trans-N-deoxyribosylase; nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase

Substrates

2-Deoxynucleoside donor; purine or pyrimidine nucleobase acceptor

Reaction

2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1

Notes

Mesophilic with a broad substrate spectrum. Recombinant protein is produced in E. coli and purified by His6-tag affinity chromatography.

Protocol

50 mM MES, pH 6
1 mM deoxythymidine
1 mM adenine
0.1 g/L enzyme
40°C, 60 min

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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