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Luciferase from Photinus pyralis (firefly), Recombinant
Cat No.
NATE-0424
Description
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Abbr
Luciferase, Recombinant (Photinus pyralis (firefly))
Alias
Luciferase
Source
E. coli
Species
Photinus pyralis (firefly)
Applications
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
Form
Colorless to pale yellow clear liquid
Enzyme Commission Number
EC 1.13.12.7
Activity
≥2.0 × 1011 RLU/mg protein
CAS No.
61970-00-1
Molecular Weight
62 kDa
Unit Definition
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
Storage
-20°C
Synonyms
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing); firefly luciferase; luciferase (firefly luciferin); Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing); firefly luciferin luciferase; Photinus pyralis luciferase; EC 1.13.12.7; 61970-00-1
Download
Download Datasheet"Luciferase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0422 | Native Photinus pyralis (firefly) Luciferase | EC 1.13.12.7 | 9014-00-0 | Photinus pyralis (firefly) | Inquiry |
| NATE-0423 | Native Vibrio fischeri (Photobacterium f) Luciferase | EC 1.13.12.7 | 9014-00-0 | Vibrio fischeri (Photobacterium f) | Inquiry |
| NATE-1253 | Luciferase from E. coli, Recombinant | EC 1.13.12.7 | E. coli | Inquiry | |
| NATE-1254 | Biotinylated Luciferase from E. coli, Recombinant | EC 1.13.12.7 | E. coli | Inquiry |