Mesophilic Bacterial PPK2 Class I Polyphosphate Kinase

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Cat No.

CEGZ-6411

Description

A recombinant bacterial class I PPK2 polyphosphate kinase designed for polyphosphate-driven phosphorylation of nucleoside diphosphates.

Abbr

PPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro ATP regeneration and phosphorylation of nucleoside diphosphates using polyphosphate as the phosphate source.

Product Overview

This PPK2 class I enzyme preferentially uses polyphosphate to phosphorylate nucleoside diphosphates and can substitute for nucleoside-diphosphate kinases or serve as an ATP regeneration catalyst in kinase reactions.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.1

CAS_No

9026-44-2

Molecular Weight

32.4 kDa

Specificity

PPK2 class I; preferential polyphosphate-driven phosphorylation of nucleoside diphosphates.

Storage

-20°C

Synonyms

PPK; PPK2 class I; polyphosphoric acid kinase; ATP:polyphosphate phosphotransferase

Substrates

ADP; polyphosphate

Reaction

ADP + (phosphate)n = ATP + (phosphate)n-1

Notes

Mesophilic enzyme suitable for ATP regeneration. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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