Mesophilic Bacterial PPK2 Class III Polyphosphate Kinase, 33.4 kDa

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Cat No.

CEGZ-6414

Description

A recombinant mesophilic bacterial class III PPK2 polyphosphate kinase with a molecular mass of 33.4 kDa.

Abbr

PPK

Source

Recombinant E. coli

Species

Bacterial

Applications

For research and in vitro polyphosphate-driven NMP/NDP phosphorylation and ATP regeneration under mesophilic conditions.

Product Overview

This class III PPK2 enzyme phosphorylates nucleotide monophosphates and diphosphates using polyphosphate, can replace NMPK or NDPK activity in selected workflows, and may generate hyperphosphorylated nucleotide products.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.1

CAS_No

9026-44-2

Molecular Weight

33.4 kDa

Specificity

PPK2 class III; nucleoside monophosphates and diphosphates. Hyperphosphorylation beyond three 5'-phosphate groups may occur.

Storage

-20°C

Synonyms

PPK2 class III; polyphosphoric acid kinase; polyP kinase; ATP:polyphosphate phosphotransferase

Substrates

AMP; ADP; other nucleoside monophosphates and diphosphates; polyphosphate

Reaction

ADP + (phosphate)n = ATP + (phosphate)n-1
AMP + (phosphate)n = ADP + (phosphate)n-1

Notes

Mesophilic enzyme suitable for ATP regeneration. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM polyphosphate
0.04 g/L enzyme
37°C, 2 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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