Native Bacillus sp Chitosanase

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Cat No.

NATE-1746

Description

Chitosanase is a powdered chitosanase preparation made by submerged fermentation of a selected strain of the bacterium Bacillus sp. The enzyme catalyzes the breakdown of chitosan, a partially or completely de-acetylated derivative of chitin (β-1,4 homopolymer of N-acetyl glucosamine).

Abbr

Chitosanase, Native (Bacillus sp)

Alias

Chitosanase

Source

Bacillus sp

Applications

Chitosanase can be used for hydrolyzing chitosan(degree of de-acetylatin: 40～100%). Especially, it can be used for the production of chitosan oligosaccharides from chitosan, which have a variety of biological activities such as immuno-stimulating activity, anti-tumor activity, anti-microbial activity, etc.

Appearance

White or light yellow colored, freeze-dried powder

Enzyme Commission Number

EC 3.2.1.132

Activity

35,000U/g

CAS_No

51570-20-8

Molecular Weight

45,000Da estimated by SDS-PAGE

pH Stability

Stable in pH range of 4.5 to 8.0

Specificity

Shows high activities against chitosan substrates which are de-acetylated by 40 to 100%.

Unit Definition

The standard activity is determined by modified Schales method. One unit(U) is defined as the amount of enzyme that releases one ㎛ole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.0 at 48°C. A detailed description of the method is available on request.

Optimum pH

pH range of 4.5 to 6.0

Optimum temperature

60°C

Thermal stability

More than 90% activity remains after 24 hr incubation at 40°C.

Storage

The product should be stored in a cool, dry environment with temperatures below 4°C.

Synonyms

Chitosanase; EC 3.2.1.132; 51570-20-8; Chitosan N-acetylglucosaminohydrolase

Notes

Arsenic: Less than 4ppm.
Heavy metals: Less than 10ppm (as Pb).
Coli-form bacteria: Less than 30 colony-forming units(CFU) per gram.
Samonella: Negative.

"Chitosanase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
CHIC-001	Chitosanase from Bacillus sp. (food grade)				Inquiry
NATE-0125	Native Streptomyces griseus Chitosanase	EC 3.2.1.132	51570-20-8	Streptomyces griseus	Inquiry
NATE-1375	Chitosanase 8B from Bacillus cereus, Recombinant	EC 3.2.1.132	51570-20-8	E. coli	Inquiry
NATE-1376	Chitosanase 46A from Bacillus subtilis, Recombinant	EC 3.2.1.132	51570-20-8	E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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