Native Bacillus sphaericus 12α-Hydroxysteroid Dehydrogenase

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Cat No.

NATE-0001

Description

In enzymology, a 12alpha-hydroxysteroid dehydrogenase (EC 1.1.1.176) is an enzyme that catalyzes the chemical reaction:3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + NADP+↔ 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate + NADPH + H+. Thus, the two substrates of this enzyme are 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate and NADP+, whereas its 3 products are 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanate, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme is involved in a metabolic pathway that degrades bile acids into cholesterol.

Abbr

12α-Hydroxysteroid Dehydrogenase, Native (Bacillus sphaericus)

Source

Bacillus sphaericus

Form

lyophilized powder

Enzyme Commission Number

EC 1.1.1.176

Activity

150-350 units/mg protein (Lowry)

CAS_No

61642-40-8

Unit Definition

One unit will oxidize 1.0 μmole of deoxycholic acid to 12-ketodeoxycholic acid per min at pH 8.0 at 37°C.

Storage

−20°C

Synonyms

EC 1.1.1.176; 61642-40-8; 12α Hydroxysteroid Dehydrogenase; 12alpha-hydroxy steroid dehydrogenase; NAD+-dependent 12alpha-hydroxysteroid dehydrogenase; NADP+-12alpha-hydroxysteroid dehydrogenase; 12-α-Hydroxysteroid Dehydrogenase; 12alpha-hydroxysteroid:NADP+ 12-oxidoreductase

"12α-Hydroxysteroid Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
EXWM-0079	12α-hydroxysteroid dehydrogenase	EC 1.1.1.176	61642-40-8		Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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