Native Bovine β(1-3,4)-Galactosidase

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Cat No.

NATE-0973

Description

Hydrolyzes non-reducing terminal galactose β(1-3) and β(1-4) linkages. Can be used in conjunction with other β-galactosidases for exoglycosidase sequencing.

Abbr

GLB, Native (Bovine)

Alias

β-gal; GLB

Source

Bovine testis

Species

Bovine

Applications

The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of β(1-3) and β(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal β(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards β(1-3) and β(1-4)-linked galactose contrasts with that of our other β-galactosidases which exhibit a preference for Gal β(1-4), and cleave the Gal β(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing β galactose residues.

Form

20 mM sodium Citrate phosphate, 150 mM NaCl (pH 4.0)

Molecular Weight

~68 kD

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyze 1 μmole of pNP-β-D-galactopyranoside per minute at pH 4.0 and 37°C.

Optimum pH

Buffer

5X concentrated buffer which when diluted gives 100 mM sodium Citrate/phosphate pH 4.0.

Synonyms

β-galactosidase; beta-gal; β-gal; lactase; β-lactosidase; maxilact; hydrolact; β-D-lactosidase; lactozym; trilactase; β-D-galactanase; oryzatym; sumiklat; β-D-galactoside galactohydrolase

"β-gal" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1745	Native Bacillus circulans β-Galactosidase	EC 3.2.1.23	9031-110-2	Bacillus circulans	Inquiry
NATE-0295	Native Bovine β-Galactosidase	EC 3.2.1.23	9031-11-2	Bovine testes	Inquiry
DIA-189	Native Escherichia coli β-Galactosidase	EC 3.2.1.23	9031-11-2	Escherichia coli	Inquiry
DIA-220	Native Aspergillus oryzae β-Galactosidase	EC 3.2.1.23	9031-11-2	Aspergillus oryzae	Inquiry

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