Native Bovine Ribonuclease A

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Cat No.

NATE-0655

Description

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2'-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds.

Abbr

RNase A, Native (Bovine)

Alias

RNase; RNase I; RNase A

Source

Bovine pancreas

Species

Bovine

Applications

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

Product Overview

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.

Form

Type I, solid; Type II, Type VI, Type VII, lyophilized powder; Type III, powder; Type IV, buffered aqueous solution, Solution in 0.2 M sodium phosphate buffer, pH 6.4; Type V, powder, white.

Enzyme Commission Number

EC 3.1.27.5

Activity

Type I, 50-150 Kunitz units/mg solid; Type II, 85-140 Kunitz units/mg protein; Type III, > 50 Kunitz units/mg protein; Type IV, > 80 Kunitz units/mg protein; Type V, ~100 units/mg protein; Type VI, 50-100 Kunitz units/mg protein; Type VII, 75-125 Kunitz units/mg protein.

CAS_No

9001-99-4

Contaminants

salt, essentially free

Molecular Weight

mol wt ~13.7 kDa

Storage

−20°C

Warnings

Chromatographically purified. Protein determined by E1%/280. RNase A purity determined by SDS-PAGE.

Synonyms

Pancreatic ribonucleases; EC 3.1.27.5; RNase; RNase I; RNase A; pancreatic RNase; ribonuclease I; endoribonuclease I; ribonucleic phosphatase; alkaline ribonuclease; ribonuclease; gene S glycoproteins; Ceratitis capitata alkaline ribonuclease; SLSG glycoproteins; gene S locus-specific glycoproteins; S-genotype-asssocd. glycoproteins; ribonucleate 3'-pyrimidino-oligonucleotidohydrolase; 9001-99-4

"Rnase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1913	Ribonuclease from Rana Pipiens, Recombinant			E. coli	Inquiry
NATE-1930	Ribonuclease T2 from Aspergillus oryzae, Recombinant	EC 3.1.27.1		Pichia pastoris	Inquiry
NATE-0656	Native Bovine Ribonuclease B	EC 3.1.27.5	9001-99-4	Bovine pancreas	Inquiry
NATE-0657	Ribonuclease H from Escherichia coli, Recombinant	EC 3.1.4.34	9050-76-4	E. coli	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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