Native Canavalia ensiformis β-N-Acetylglucosaminidase

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Cat No.

NATE-0780

Description

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

Abbr

β-N-Acetylglucosaminidase, Native (Canavalia ensiformis)

Source

Canavalia ensiformis

Applications

β-N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease 1. Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms.

Product Overview

At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.

Form

ammonium sulfate suspension. Suspension in 2.5 M (NH4)2SO4, pH 7.0

Enzyme Commission Number

EC 3.2.1.52

Activity

> 15 units/mg protein

CAS_No

9012-33-3

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at pH 5.0 at 25°C.

Storage

2-8°C

Synonyms

hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase; EC 3.2.1.52; 9012-33-3

"β-N-Acetylhexosaminidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1290	β-acetylglucosaminidase 73A from Lactococcus lactis, Recombinant			E. coli	Inquiry
NATE-1220	β-N-Acetylhexosaminidase from Streptococcus pyogenes, Recombinant	EC 3.2.1.52	9012-33-3	Streptococcus pyogenes M1 GAS SF370	Inquiry
NATE-1289	β-acetylglucosaminidase 73A from Clostridium perfringens, Recombinant			E. coli	Inquiry
NATE-0781	Native Human β-N-Acetylglucosaminidase	EC 3.2.1.52	9012-33-3	Human placenta	Inquiry

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