Native Cellulomonas sp. Glycerokinase

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Cat No.

NATE-0287

Description

Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.

Abbr

GK, Native (Cellulomonas sp.)

Alias

Source

Cellulomonas sp.

Applications

This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.

Product Overview

The enzyme has the highest specificity for glycerol, and also phosphorylates dihydroxyacetone and glyceraldehyde. Mg++ is essentially required for the reaction.

Form

Lyophilized powder containing phosphate buffer salts and sodium gluconate

Enzyme Commission Number

EC 2.7.1.30

Activity

20 U/mg-solid or more

CAS_No

9030-66-4

Molecular Weight

mol wt ~128 kDa ((by gel filtration)

Isoelectric point

4.2

pH Stability

pH 5.5 x 10.0 (25°C, 20hr)

Michaelis Constant

4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)

Unit Definition

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH.

Optimum pH

9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)

Optimum temperature

50°C

Thermal stability

below 40°C (pH 7.5, 15min)

Storage

−20°C

Inhibitors

p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)

Synonyms

EC 2.7.1.30; glycerokinase; GK; ATP:glycerol-3-phosphotransferase; glycerol kinase (phosphorylating); glyceric kinase; 9030-66-4

"GK" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-149	Native Microorganism Glycerol Kinase	EC 2.7.1.30	9030-66-4	Microorganism	Inquiry
NATE-0286	Native Bacillus stearothermophilus Glycerokinase	EC 2.7.1.30	9030-66-4	Bacillus stearothermophilus	Inquiry
NATE-0288	Native Escherichia coli Glycerokinase	EC 2.7.1.30	9030-66-4	Escherichia coli	Inquiry
NATE-0289	Native Streptomyces canus Glycerokinase	EC 2.7.1.30	9030-66-4	Streptomyces canus	Inquiry

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Project Description:

Our Products Cannot Be Used As Medicines Directly For Personal Use.

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