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Native E. coli Inorganic Pyrophosphatase

Cat No.
NATE-1272
Description
Pyrophosphatase (or inorganic pyrophosphatase) is an enzyme (EC 3.6.1.1) that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis,as well as other biochemical transformations.
Abbr
Inorganic pyrophosphatase, Native (E. coli)
Source
E. coli
Applications
Increasing RNA yield in transcription reaction; enhancing DNA replication.
Form
20 mM Tris-HCl, 100 mM NaCl, 1 mM Dithiothreitol, 0.1 mM EDTA, 50% Glycerol, pH 8.0 25°C. Store at –20°C.
CAS No.
9024-82-2
Concentration
100 units/ml
Unit Definition
One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2 and 2 mM PPi).
Synonyms
Pyrophosphate phosphohydrolase; inorganic pyrophosphatase; EC 3.6.1.1; 9024-82-2; iphosphate phosphohydrolase

"Inorganic pyrophosphatase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1843 Inorganic Diphosphatase (Crude Enzyme) EC 3.6.1.1 9024-82-2 E. coli Inquiry
NATE-0353 Native Bacillus stearothermophilus Inorganic Pyrophosphatase EC 3.6.1.1 9024-82-2 Bacillus stearothermophilus Inquiry
NATE-0354 Native Baker's yeast (S. cerevisiae) Inorganic Pyrophosphatase EC 3.6.1.1 9024-82-2 Baker's yeast (S. cerevisiae) Inquiry
NATE-0355 Inorganic Pyrophosphatase from Escherichia coli, Recombinant EC 3.6.1.1 9024-82-2 E. coli Inquiry

Our Products Cannot Be Used As Medicines Directly For Personal Use.

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