Native Gluconobacter industrius D-Fructose Dehydrogenase

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Cat No.

NATE-0184

Description

D-fructose dehydrogenase is a heterotrimeric membrane-bound enzyme commonly seen in various Gluconobacter sp. especially in Gluconobacter japonicus (Gluconobacter industrius). It has a molecular mass of ca. 140 kDa, consisting of subunits I (67kDa), II (51 kDa), and III (20 kDa) and catalyzes the oxidation of D-fructose to produce 5-keto-D-fructose. The enzyme is a flavoprotein-cytochrome c complex with subunits I and II covalently bound to flavin adenine dinucleotide (FAD) and heme C as prosthetic groups, respectively.

Abbr

D-Fructose Dehydrogenase, Native (Gluconobacter industrius)

Source

Gluconobacter industrius

Applications

D-fructose dehydrogenase is used as a biosensor to detect the presence of D-fructose. Fructose dehydrogenase (FDH) is used in a number of basic research projects to examine the electrochemical properties of enzyme-catalyzed electrode reactions called bioelectrocatalysis. D-fructose dehydrogenase has been used in a study that contributed towards a convenient method for measuring rare sugars, monosaccharides, for applications in the bio-industry. A direct electron transfer reaction of d-fructose dehydrogenase adsorbed on a porous carbon electrode surface has been used to describe a batch-type coulometric d-fructose biosensor.

Form

Lyophilized powder

Enzyme Commission Number

EC 1.1.99.11

Activity

400-1,200 units/mg

CAS_No

37250-85-4

Composition

Protein, <10% Lowry

Unit Definition

One unit will convert 1.0 μmole D-fructose to 5-ketofructose per min at pH 4.5 at 37°C.

Storage

−20°C

Synonyms

EC 1.1.99.11; fructose 5-dehydrogenase; D-fructose dehydrogenase; D-fructose:(acceptor) 5-oxidoreductase; 37250-85-4

"D-Fructose Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0185	Native Gluconobacter sp. D-Fructose Dehydrogenase	EC 1.1.99.11	37250-85-4	Gluconobacter sp.	Inquiry
EXWM-0444	fructose 5-dehydrogenase	EC 1.1.99.11	37250-85-4		Inquiry

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