Description
In enzymology, a ADP-Dependent Hexokinase (EC 2.7.1.147) is an enzyme that catalyzes the chemical reaction: D‒Glucose + ADP → D‒Glucose‒6‒phosphate + AMP.
Abbr
Hexokinase (ADP-Dependent), Native (Pyrococcus furiosus)
Source
Pyrococcus furiosus
Applications
Useful for the enzymatic determination of ADP.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 2.7.1.147
Activity
More than 30 U/mg solid
Contaminants
NADPH oxidase < 0.01%; ATPase < 0.01%
Molecular Weight
100 kDa (gel filtration) 51 kDa (SDS‒PAGE)
Michaelis Constant
Glucose 0.64 mM (at 37°C) ADP 0.07 mM (at 37°C)
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of glucose to D‒Glucose‒6‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum temperature
100°C
Activators
Mg2+, Co2+, Mn2+
Thermal stability
Stable at 95°C and below
Storage
Storage at －20°C in the presence of a desiccant is recommended.
Synonyms
ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 2.7.1.147
