Native Microorganism Glucose Dehydrogenase (NAD(P)-dependent)

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Cat No.

DIA-191

Description

In enzymology, a glucose 1-dehydrogenase (EC 1.1.1.47) is an enzyme that catalyzes the chemical reaction: beta-D-glucose + NAD(P)+ ↔ D-glucono-1,5-lactone + NAD(P)H + H+. The 3 substrates of this enzyme are beta-D-glucose, NAD+, and NADP+, whereas its 4 products are D-glucono-1,5-lactone, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

Abbr

Glucose Dehydrogenase (Microorganism)

Source

Microorganism

Applications

This enzyme is useful for enzymatic determination of D-Glucose.

Appearance

White amorphous powder, lyophilized

Form

Freeze dried powder

Enzyme Commission Number

EC 1.1.1.47

Activity

GradeⅢ 250U/mg-solid or more

CAS_No

9028-53-9

Contaminants

NADH oxidase < 1.0×10⁻³% α-Glucosidase < 1.0×10⁻³% Glucose-6-phosphate dehydrogenase < 1.0×10⁻³%

Molecular Weight

approx. 101 kDa (Gel filtration)

Isoelectric point

4.5

pH Stability

pH 6.0-7.5 (20°C, 16hr)

Michaelis Constant

NAD⁺linked : 1.38×10⁻²M (D-Glucose) 3.09×10⁻⁴M (NAD⁺), NADP⁺linked : 1.25×10⁻²M (D-Glucose) 4.07×10⁻⁵M (NADP⁺)

Specificity

Specific for ß-D,-Glucose or 2-Deoxy-glucose (Either NAD⁺ or NADP⁺ serves as coenzyme.)

Optimum pH

Optimum temperature

55°C

Thermal stability

45°C (15min-treatment with 50mM K-phosphate buffer, pH 7.0)

Stability

Stable at-20°C for at least one year

Inhibitors

Ag⁺, Hg²⁺, Monoiodoacetate

Synonyms

Glucose Dehydrogenase; EC 1.1.1.47; beta-D-glucose: NAD(P)+ 1-oxidoreductase; D-glucose dehydrogenase (NAD(P)+)

"Glucose Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1794	Glucose Dehydrogenase (Crude Enzyme)	EC 1.1.1.47	9028-53-9	E. coli	Inquiry
NATE-1902	Glucose Dehydrogenase from E. coli, Recombinant	EC 1.1.1.47	9028-53-9	E. coli	Inquiry
DIA-192	Native Microorganism Glucose Dehydrogenase (PQQ-dependent)	EC 1.1.5.2	81669-60-5	Microorganism	Inquiry
NATE-0305	Native Pseudomonas sp. Glucose dehydrogenase	EC 1.1.1.47	9028-53-9	Pseudomonas sp.	Inquiry

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