Native Proteus sp. L-Glutamic Dehydrogenase (NADP)

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Cat No.

NATE-0395

Description

L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.

Abbr

L-Glutamic Dehydrogenase, Native (Proteus sp.)

Source

Proteus sp.

Applications

This enzyme is useful for enzymatic determination of NH3, α-ketoglutaric acid and L-glutamic acid, and for assay of leucine aminopeptidase and urease. This enzyme is also used for enzymatic determination of urea when coupled with urease (URH-201) in clinical analysis.

Form

buffered aqueous solution; Solution in 50 mM Tris HCl, pH 7.8, 5 mM Na2EDTA containing 0.05% sodium azide

Enzyme Commission Number

EC 1.4.1.4

Activity

> 400 units/mg protein (biuret)

CAS_No

9029-11-2

Molecular Weight

mol wt ~300 kDa

Isoelectric point

4.6

pH Stability

pH 6.0-8.5 (25°C, 20hr)

Michaelis Constant

1.1 X 10-3M (NH3), 3.4 X 10-4M (α-Ketoglutarate) 1.2 X 10-3M (L-Glutamate), 1.4 X 10-5M (NADPH), 1.5 X 10-5M (NADP+) Structure : 6 subunits (M.W.50 kDa) per mol of enzyme

Unit Definition

One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 8.3 at 30°C in the presence of ammonium ions and NADPH.

Optimum pH

8.5 (α-KG→L-Glu) 9.8 (L-Glu→α-KG)

Optimum temperature

45°C (α-KG−L-Glu) 45-55°C (L-Glu→α-KG)

Thermal stability

below 50°C (pH 7.4, 10min)

Storage

2-8°C

Inhibitors

Hg++, Cd++, p-chloromercuribenzoate, pyridine, 4-4′-dithiopyridine, 2,2′-dithiopyridine

Warnings

Do not confuse with non-specific L-GLDH, EC 1.4.1.3.

Synonyms

L-Glutamic Dehydrogenase; EC 1.4.1.4; 9029-11-2; glutamic dehydrogenase; dehydrogenase, glutamate (nicotinamide adenine dinucleotide (phosphate)); glutamic acid dehydrogenase; L-glutamate dehydrogenase; L-glutamic acid dehydrogenase; NAD (P)-glutamate dehydrogenase; NAD (P)H-dependent glutamate dehydrogenase; glutamate dehydrogenase (NADP)

"GLDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1802	L-Glutamate Dehydrogenase (Crude Enzyme)	EC 1.4.1.2	9001-46-1	E. coli	Inquiry
NATE-1701	Glutamate Dehydrogenase from Thermophilic Bacterium, recombinant	EC 1.4.1.2	9001-46-1	E. coli	Inquiry
NATE-1037	Native Glutamate dehydrogenase (NADP+) from Yeast	EC 1.4.1.4	2604121	Yeast	Inquiry
NATE-1145	Glutamate dehydrogenase, Recombinant	EC 1.4.1.2	9001-46-1		Inquiry

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