Native Pseudomonas sp. Lipoprotein lipase

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Cat No.

DIA-210

Description

Lipoprotein lipase (LPL) (EC 3.1.1.34) is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids.

Abbr

LPL (Pseudomonas sp.)

Alias

LPL

Source

Pseudomonas sp.

Applications

This enzyme is useful for enzymatic determination of triglyceride in serum when coupled with L-α-glycerophosphate oxidase and glycerol kinase. Usually, the reaction can be completed in 5 minutes at 37°C by using 2.5~3.0 units of the enzyme per test (3.0ml) at pH around 7.0.

Appearance

Light brown amorphous powder, lyophilized

Enzyme Commission Number

EC 3.1.1.34

Activity

GradeⅢ　20U/mg-solid or more (containing approx. 80% of stabilizers)

CAS_No

9004-02-8

Contaminants

Phosphatase < 1.0×10⁻³% Catalase < 2.0×10⁻²% NADH oxidase< 1.0×10⁻³% Cholesterol oxidase < 2.0×10⁻³%

Molecular Weight

approx. 134 kDa

Isoelectric point

5.95±0.05

pH Stability

pH 7.0-9.0 (25°C, 20hr)

Optimum pH

7.0-9.0

Optimum temperature

45-50°C

Thermal stability

below 55°C (pH 7.0, 10min)

Stability

Stable at-20°C for at least one year

Stabilizers

Mg⁺⁺, Na-cholate, bovine serum albumin

Inhibitors

Hg⁺⁺, Ag⁺, ionic detergents

Synonyms

Lipoprotein lipase; LPL; EC 3.1.1.34; Clearing factor lipase; Diacylglycerol lipase; Diglyceride lipase

"LPL" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-211	Native Microorganism Lipoprotein lipase	EC 3.1.1.34	9004-02-8	Microorganism	Inquiry
DIA-282	Chemically modified Pseudomonas species Lipoprotein Lipase			Pseudomonas species	Inquiry
NATE-0416	Native Bovine Lipoprotein Lipase	EC 3.1.1.34	9004-02-8	Bovine milk	Inquiry
NATE-0417	Native Burkholderia sp. Lipoprotein Lipase	EC 3.1.1.34	9004-02-8	Burkholderia sp.	Inquiry

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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