Native T4-infected Escherichia coli Polynucleotide Kinase

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Cat No.

NATE-0605

Description

Polynucleotide kinase catalyses a "forward reaction" transfer of the γ-phosphate of ATP to the 5' hydroxyl terminus of single-and double-stranded nucleic acids (DNA and RNA) and 3'-nucleoside monophosphates. In exchange reactions containing ADP, the enzyme will catalyze the exchange of 5'-terminal phosphate groups and ATP. The 3'-phosphatase activity enables the enzyme to remove 3'-phosphoryl groups from phosphorylpolynucleotides.

Abbr

PNK, Native (T4-infected Escherichia coli)

Alias

PNK; DNA kinase

Source

T4-infected Escherichia coli

Applications

Suitable for: • Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling • 5′ phosphorylation of oligonucleotides • Removal of 3′-phosphate groups from phosphorylpolynucleotides

Form

buffered aqueous glycerol solution

Enzyme Commission Number

EC 2.7.1.78

Activity

10 units/μL

CAS_No

37211-65-7

Molecular Weight

mol wt 33 kDa

Concentration

10 units/μL

Unit Definition

One unit catalyzes the transfer of one nanomole of 32P to the 5'-end of micrococcal nuclease-treated DNA in 30 min. at 37°C. Transfer is detected as incorporation into acid-insoluble material.

Storage

−20°C

Warnings

Activity is determined in a reaction mixture containing 40 mM Tris-HCl (pH 7.5), with 10 mM MgCl2, 5 mM dithiothreitol, 0.5 mM 5’-OH polynucleotide ends, and mM [γ-32P]-ATP.

Synonyms

polynucleotide 5'-hydroxyl-kinase; EC 2.7.1.78; 37211-65-7; ATP:5'-dephosphopolynucleotide 5'-phosphatase; PNK; polynucleotide 5'-hydroxyl kinase (phosphorylating); 5'-hydroxyl polynucleotide kinase; 5'-hydroxyl polyribonucleotide kinase; 5'-hydroxyl RNA kinase; DNA 5'-hydroxyl kinase; DNA kinase; polynucleotide kinase; polynucleotide 5'-hydroxy-kinase

"PNK" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
EXWM-3107	polynucleotide 5'-hydroxyl-kinase	EC 2.7.1.78	37211-65-7		Inquiry

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