Products

Enzymes for Research, Diagnostic and Industrial Use

Nitrile hydratase from Rhodopseudomonas palustris, Recombinant

Cat No.
NATE-1218
Description
In enzymology, nitrile hydratases (NHases; EC 4.2.1.84) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of diverse nitriles to their corresponding amides: R-C≡N + H2O → R-C(O)NH2.
Abbr
Nhase, Recombinant (Rhodopseudomonas palustris)
Alias
Nhase
Source
Rhodopseudomonas palustris CGA009
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.1.84
Activity
21.97 U/mg
CAS_No
82391-37-5
Molecular Weight
25878.9 Da (α-subunit), 24204.3 Da (β-subunit)
Purity
> 95 % as judged by SDS-PAGE
Concentration
112.05 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of methacrylamide from methocrylonitrile (10 mM) per minute in 100 mM KH2PO4 buffer, pH 7.0, at 25°C, as measured at 224 nm.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase; aliphatic-amide hydro-lyase (nitrile-forming)

"Nhase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-1852 Nitrile Hydratase (Crude Enzyme) EC 4.2.1.84 82391-37-5 E. coli Inquiry
NATE-1217 Nitrile hydratase from Rhodococcus erythropolis, Recombinant EC 4.2.1.84 82391-37-5 Rhodococcus erythropolis AJ270 Inquiry
NATE-1219 Nitrile hydratase from Sinorhizobium meliloti, Recombinant EC 4.2.1.84 82391-37-5 Sinorhizobium meliloti 1021 Inquiry
NATE-1626 Nitrile hydratase from Recombinant microoganism EC 4.2.1.84 82391-37-5 Inquiry

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