Pectin acetylesterase from Clostridium thermocellum, Recombinant

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Cat No.

NATE-1224

Description

Pectin acetylesterase catalyses the hydrolysis of acetate from acetylated pectins.

Abbr

PAE, Recombinant (Clostridium thermocellum)

Alias

PAE

Source

Clostridium thermocellum

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 3.1.1.-

Activity

100 U/mg

Molecular Weight

23800 Da

Purity

> 95 % as judged by SDS-PAGE

Concentration

200 U/ml

Unit Definition

One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per minute from p-nitrophenyl acetate (1 mM in the assay) in 50 mM phosphate buffer, pH 6.5, at 50°C, containing 1 mg/ml of BSA.

Optimum pH

6.5 (stable from 6 – 7.5)

Optimum temperature

50°C (stable up to 60°C)

Storage

Store at 4°C (shipped at room temperature)

Preparation Instructions

Agitate vial sufficiently to fully homogenise enzyme precipitate before use.

Synonyms

Pectinacetylesterase; PAE

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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