Leaf-branch Compost Poly (ethylene terephthalate) Hydrolase

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Cat No.

EXWM-3437

Description

PETases are an esterase class of enzymes that catalyze the hydrolysis of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl terephthalate (MHET). The idealized chemical reaction is (where n is the number of monomers in the polymer chain):
(ethylene terephthalate)n + H2O → (ethylene terephthalate)n-1 + MHET
Trace amount of the PET breaks down to bis(2-hydroxyethyl) terephthalate (BHET). PETases can also break down PEF-plastic (polyethylene-2,5-furandicarboxylate), which is a bioderived PET replacement. PETases can't catalyze the hydrolysis of aliphatic polyesters like polybutylene succinate or polylactic acid.
Non-enzymatic natural degradation of PET will take hundreds of years, but PETases can degrade PET in matter of days.

Source

E. coli

Form

Freeze-dried powder

Enzyme Commission Number

EC 3.1.1.101

Activity

194U/mg

Molecular Weight

27.6 kDa

Purity

>95% by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme that will generate 1.0 mmol TNB per minute at 50 °C.

Storage

Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Buffer

20mM Tris, pH8.0, 300mM NaCl, 200mM imidazole and 3% trehalose

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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