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Pullulanase from Bacillus subtilis, Recombinant

Cat No.
NATE-1227
Description
Pullulanase is a lipoprotein generated as a precursor containing a 19-amino acid signal peptide followed by a palmitate-modified cysteine residue. The signal peptide gets cleaved prior to secretion into the extracellular matrix.
Abbr
Pullulanase, Recombinant (Bacillus subtilis)
Source
Bacillus subtilis subsp. subtilis str. 168
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.41
Activity
84.66 U/mg
CAS No.
9075-68-7
Molecular Weight
84420.4 Da
Purity
>95 % as judged by SDS-PAGE
Concentration
558.77 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of D-glucose equivalents per minute from soluble starch (9.04 mg/mL; ACS reagent; solubilised by boiling for 5 min in H2O) in 22.59 mM sodium acetate buffer, pH 5.0, containing 0.452 mg/mL BSA, at 37°C.
Optimum pH
~ 5.0
Optimum temperature
> 37°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Pullulanase; EC 3.2.1.41; limit dextrinase (erroneous); amylopectin 6-glucanohydrolase; bacterial debranching enzyme; debranching enzyme; α-dextrin endo-1,6-α-glucosidase; R-enzyme; pullulan α-1,6-glucanohydrolase; 9075-68-7; Pullulanase M2

"Pullulanase" Total Products Page

Catalog Product Name EC No. CAS No. Source Price
NATE-0643 Native Klebsiella pneumoniae Pullulanase EC 3.2.1.41 9075-68-7 Klebsiella pneumoniae Inquiry
NATE-1448 Pullulanase 13A from Thermotoga maritima, Recombinant EC 3.2.1.41 9075-68-7 E. coli Inquiry
BRE-1618 Pullulanase for Saccharification 9075-68-7 Inquiry
SUG-003 Pullulanase (Food Grade) 9075-68-7 Bacillus licheniformis Inquiry
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