Pyroglutamate Aminopeptidase from Pyrococcus furiosus, Recombinant

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Cat No.

NATE-0648

Description

Pyroglutamate aminopetidase is an enzyme that digests proteins. This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100°C and the optimal pH range is 6.0 to 9.0.

Abbr

Pyrase, Native (Pyrococcus furiosus)

Alias

Pyrase

Source

E. coli

Species

Pyrococcus furiosus

Applications

Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides. The enzyme from Creative Enzymes has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin.

Product Overview

recombinant, expressed in E. coli, ~90% (SDS-PAGE)

Form

Lyophilized powder containing sodium phosphate

Enzyme Commission Number

EC 3.4.19.3

Activity

> 0.11 units/mg protein

CAS_No

9075-21-2

Molecular Weight

mol wt 24.072 kDa by amino acid sequence mol wt 28 kDa by SDS-PAGE

Unit Definition

One unit will hydrolyze 1 μmol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37°C.

Optimum pH

6.0 to 9.0

Optimum temperature

95 to 100°C

Storage

−20°C

Synonyms

pyroglutamyl-peptidase I; Pyroglutamate aminopeptidase; EC 3.4.19.3; 5-oxoprolyl-peptidase; pyrase; pyroglutamate aminopeptidase; pyroglutamyl aminopeptidase; L-pyroglutamyl peptide hydrolase; pyrrolidone-carboxyl peptidase; pyrrolidone-carboxylate peptidase; pyrrolidonyl peptidase; L-pyrrolidonecarboxylate peptidase; pyroglutamidase; pyrrolidonecarboxylyl peptidase; 9075-21-2

"Pyrase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0647	Native Bacillus sp. Pyroglutamate Aminopeptidase	EC 3.4.19.3	9075-21-2	Bacillus sp.	Inquiry
NATE-1256	Native Thermococcus thioreducens Pyroglutamate Aminopeptidase			Thermococcus thioreducens	Inquiry
EXWM-4088	pyroglutamyl-peptidase I	EC 3.4.19.3	9075-21-2		Inquiry

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Project Description:

Our Products Cannot Be Used As Medicines Directly For Personal Use.

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