Recombinant E. coli Adenosine Deaminase, 37.7 kDa

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Cat No.

CEGZ-6427

Description

A recombinant E. coli adenosine deaminase that irreversibly converts adenosine and deoxyadenosine to the corresponding inosine products.

Abbr

ADA

Alias

adenosine aminohydrolase

Source

Recombinant E. coli

Species

Escherichia coli

Applications

For research and in vitro deamination of adenosine and deoxyadenosine in purine metabolism and nucleoside-conversion studies.

Product Overview

The enzyme catalyzes hydrolytic deamination of adenosine to inosine and deoxyadenosine to deoxyinosine. The preparation is a mesophilic liquid recombinant enzyme with a molecular mass of 37.7 kDa.

Form

Liquid

Enzyme Commission Number

EC 3.5.4.4

CAS_No

9026-93-1

Molecular Weight

37.7 kDa

Specificity

Adenosine and deoxyadenosine.

Storage

-20°C

Synonyms

Ec-Ada; ADA; adenosine aminohydrolase

Substrates

Adenosine; deoxyadenosine

Reaction

Adenosine + H2O + H+ = inosine + NH4+
Deoxyadenosine + H2O + H+ = deoxyinosine + NH4+

Notes

Mesophilic recombinant enzyme produced in E. coli and purified by His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

50 mM Tris-HCl, pH 7.6 (at 37°C)
5 mM adenosine
0.1 g/L enzyme
37°C, 1 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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