Recombinant E. coli MTA/SAH Nucleosidase

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Cat No.

CEGZ-6426

Description

A recombinant E. coli MTA/SAH nucleosidase that cleaves multiple sulfur-containing adenosine metabolites and selected purine ribonucleosides.

Abbr

MTAN

Alias

SAHN; MtnN

Source

Recombinant E. coli

Species

Escherichia coli

Applications

For research and in vitro hydrolysis of S-adenosylhomocysteine, 5-methylthioadenosine, 5-deoxyadenosine, and related substrates.

Product Overview

The enzyme cleaves the glycosidic bond of both MTA and SAH to release adenine and the corresponding thioribose product. It also converts 5-deoxyadenosine and accepts several related thioadenosine/adenosylhomocysteine analogs and purine ribonucleosides in vitro.

Form

Liquid

Enzyme Commission Number

EC 3.2.2.9

CAS_No

9055-10-1

Molecular Weight

25.8 kDa

Specificity

MTA, SAH/AdoHcy, 5-deoxyadenosine, selected thioadenosine and adenosylhomocysteine analogs, and purine ribonucleosides.

Storage

-20°C

Synonyms

Ec-MtnN; MTAN; SAHN; Ec-YadA; 5-methyladenosine nucleosidase; S-methyl-5-thioadenosine/S-adenosyl-L-homocysteine nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy/MTA nucleosidase

Substrates

S-adenosyl-L-homocysteine; 5-deoxyadenosine; S-methyl-5-thioadenosine; selected purine nucleosides

Reaction

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
5-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
S-methyl-5-thioadenosine + H2O = 5-(methylsulfanyl)-D-ribose + adenine
Purine nucleoside + H2O = D-ribose + purine base

Notes

Mesophilic recombinant enzyme produced in E. coli and purified by His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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