Recombinant E. coli RihA Pyrimidine Ribonucleoside Hydrolase

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Cat No.

CEGZ-6422

Description

A recombinant E. coli RihA ribonucleoside hydrolase that selectively hydrolyzes pyrimidine ribonucleosides.

Abbr

RNH

Alias

RihA; YbeK

Source

Recombinant E. coli

Species

Escherichia coli

Applications

For research and in vitro hydrolysis of cytidine, uridine, and related pyrimidine ribonucleosides to ribose and the corresponding base.

Product Overview

The enzyme catalyzes irreversible N-riboside hydrolysis and is strictly specific for ribonucleosides. Cytidine and uridine are accepted with approximately equal efficiency.

Form

Liquid

Enzyme Commission Number

EC 3.2.2.8

CAS_No

37288-60-1

Molecular Weight

35.2 kDa

Specificity

Strictly specific for ribonucleosides; cytidine and uridine are accepted with similar efficiency.

Storage

-20°C

Synonyms

Ec-RihA; RNH; Ec-YbeK; ribosylpyrimidine nucleosidase; pyrimidine nucleoside hydrolase; N-ribohydrolase; N-ribosylpyrimidine nucleosidase; pyrimidine-nucleoside ribohydrolase

Substrates

Cytidine; uridine; pyrimidine ribonucleosides

Reaction

Pyrimidine nucleoside + H2O = D-ribose + pyrimidine base

Notes

Mesophilic recombinant enzyme produced in E. coli and purified by His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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