Recombinant E. coli RihB Cytidine-Preferring Ribonucleoside Hydrolase

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Cat No.

CEGZ-6423

Description

A recombinant E. coli RihB ribonucleoside hydrolase with a clear preference for cytidine over uridine and slower activity toward purine ribonucleosides.

Abbr

RNH

Alias

RihB; YeiK

Source

Recombinant E. coli

Species

Escherichia coli

Applications

For research and in vitro hydrolysis of pyrimidine ribonucleosides where cytidine-preferring substrate selectivity is useful.

Product Overview

The enzyme is strictly specific for ribonucleosides and hydrolyzes cytidine more efficiently than uridine. Purine ribonucleosides are also accepted but are converted much more slowly.

Form

Liquid

Enzyme Commission Number

EC 3.2.2.8

CAS_No

37288-60-1

Molecular Weight

35.1 kDa

Specificity

Ribonucleosides only; cytidine > uridine, with much slower conversion of purine ribonucleosides.

Storage

-20°C

Synonyms

Ec-RihB; RNH; Ec-YeiK; ribosylpyrimidine nucleosidase; pyrimidine nucleoside hydrolase; N-ribohydrolase; N-ribosylpyrimidine nucleosidase; pyrimidine-nucleoside ribohydrolase

Substrates

Cytidine; uridine; selected purine ribonucleosides

Reaction

Pyrimidine nucleoside + H2O = D-ribose + pyrimidine base

Notes

Mesophilic recombinant enzyme produced in E. coli and purified by His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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