Recombinant E. coli RihC Broad-Spectrum Ribonucleoside Hydrolase

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Cat No.

CEGZ-6424

Description

A recombinant E. coli RihC hydrolase with broad activity toward both purine and pyrimidine ribonucleosides.

Abbr

RNH

Alias

RihC; YaaF

Source

Recombinant E. coli

Species

Escherichia coli

Applications

For research and in vitro hydrolysis of diverse ribonucleosides to ribose and the corresponding purine or pyrimidine base.

Product Overview

This enzyme hydrolyzes both purine and pyrimidine ribonucleosides and is strictly specific for ribonucleosides. The reported activity order is uridine > xanthosine > inosine > adenosine > cytidine > guanosine.

Form

Liquid

Enzyme Commission Number

EC 3.2.2.1 / EC 3.2.2.8

CAS_No

9025-44-9 / 37288-60-1

Molecular Weight

34.0 kDa

Specificity

Purine and pyrimidine ribonucleosides; activity order: uridine > xanthosine > inosine > adenosine > cytidine > guanosine.

Storage

-20°C

Synonyms

Ec-RihC; RNH; Ec-YaaF; non-specific ribonucleoside hydrolase; purine-nucleoside ribohydrolase

Substrates

Uridine; xanthosine; inosine; adenosine; cytidine; guanosine

Reaction

Purine nucleoside + H2O = D-ribose + purine base
Pyrimidine nucleoside + H2O = D-ribose + pyrimidine base

Notes

Mesophilic recombinant enzyme produced in E. coli and purified by His-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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