Recombinant Human NME2 Nucleoside-Diphosphate Kinase

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Cat No.

CEGZ-6410

Description

A recombinant human NME2 nucleoside-diphosphate kinase that catalyzes phosphate transfer from nucleoside triphosphates to nucleoside diphosphates.

Abbr

NDPK

Alias

NME2

Source

Recombinant E. coli

Species

Homo sapiens

Applications

For research and in vitro nucleotide interconversion studies involving nucleoside diphosphate and triphosphate pools.

Product Overview

The enzyme catalyzes ATP-dependent phosphorylation of nucleoside diphosphates to the corresponding nucleoside triphosphates and exhibits broad substrate acceptance.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.6

CAS_No

9026-51-1

Molecular Weight

18.7 kDa

Specificity

Broad substrate spectrum for nucleoside diphosphates.

Storage

-20°C

Synonyms

hNME2; NDPK; nucleoside 5'-diphosphate kinase; nucleoside diphosphokinase; nucleotide phosphate kinase; NDP kinase; ATP:nucleoside-diphosphate phosphotransferase

Substrates

ATP; nucleoside diphosphates

Reaction

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

Notes

Mesophilic enzyme with a broad substrate spectrum. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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