Recombinant Human UMP/CMP Kinase CMPK1

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Cat No.

CEGZ-6408

Description

A recombinant human CMPK1 enzyme that functions as a bifunctional UMP/CMP kinase in pyrimidine nucleotide metabolism.

Abbr

CMPK1

Alias

U/CMPK; UCK

Source

Recombinant E. coli

Species

Homo sapiens

Applications

For research and in vitro phosphorylation of CMP, UMP, and dCMP in studies of pyrimidine nucleotide biosynthesis and salvage.

Product Overview

The enzyme phosphorylates CMP and UMP with similar efficiency and can also accept dCMP. It participates in de novo pyrimidine nucleotide biosynthesis.

Form

Liquid

Enzyme Commission Number

EC 2.7.4.14

CAS_No

37278-21-0

Molecular Weight

23.6 kDa

Specificity

CMP and UMP with similar efficiency; dCMP is also accepted.

Storage

-20°C

Synonyms

hCMPK1; U/CMPK; UCK; cytidylate kinase; cytidine monophosphate kinase; CMP kinase; CTP:CMP phosphotransferase; deoxycytidylate kinase; deoxycytidine monophosphate kinase; dCMP kinase; uridylate kinase; uridine monophosphate kinase; pyrimidine nucleoside monophosphate kinase; ATP:UMP-CMP phosphotransferase

Substrates

ATP; CMP; UMP; dCMP

Reaction

ATP + (d)CMP = ADP + (d)CDP
ATP + UMP = ADP + UDP

Notes

Mesophilic enzyme. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/L enzyme
37°C, overnight

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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