Recombinant Human Uridine/Cytidine Kinase UCK2

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Cat No.

CEGZ-6398

Description

A recombinant human UCK2 isoform 1 enzyme that phosphorylates pyrimidine ribonucleosides with defined selectivity against deoxyribonucleosides and purine ribonucleosides.

Abbr

UCK2

Alias

UCK; URK1

Source

Recombinant E. coli

Species

Homo sapiens

Applications

For research and in vitro phosphorylation of uridine, cytidine, and related pyrimidine ribonucleoside analogs to their monophosphate forms.

Product Overview

The enzyme converts uridine and cytidine to UMP and CMP, respectively, using ATP as phosphate donor. It does not phosphorylate deoxyribonucleosides or purine ribonucleosides.

Form

Liquid

Enzyme Commission Number

EC 2.7.1.48

CAS_No

9026-39-5

Molecular Weight

30.7 kDa

Specificity

Uridine, cytidine, and related pyrimidine ribonucleoside analogs; does not phosphorylate deoxyribonucleosides or purine ribonucleosides.

Storage

-20°C

Synonyms

hUCK2; UCK; URK1; pyrimidine ribonucleoside kinase; uridine-cytidine kinase; uridine kinase; uridine phosphokinase; ATP:uridine 5'-phosphotransferase; ATP:uridine/cytidine 5'-phosphotransferase

Substrates

ATP; uridine; cytidine

Reaction

ATP + uridine = ADP + UMP
ATP + cytidine = ADP + CMP

Notes

Mesophilic enzyme with broad acceptance of pyrimidine ribonucleotides. Recombinant protein produced in E. coli and purified by His6-tag affinity chromatography. Optimal reaction time and enzyme concentration may vary depending on the substrate.

Protocol

70 mM Tris-HCl, pH 7.6 (at 37°C)
10 mM MgCl2
5 mM uridine
6 mM ATP
0.04 g/L enzyme
37°C, 1 h

Usage

Research and in vitro use only.

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Project Description:

For research and industrial use only. Not intended for personal medicinal use. Certain food-grade products are suitable for formulation development in food and related applications.

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